other:Article Title: Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts
Article Snippet: The primary antibodies and dilutions used were: Mouse anti-CDX2 (1:100; BioGeneX, MU392A-5UC), Rabbit anti-CDX2 (1:100; Abcam, ab76541), Rabbit anti-SOX2 (1:200; Abcam, ab97959), Mouse anti-GATA2 (1:50; Santa Cruz Biotechnology, sc-267), Goat anti-GATA6 (1:200; R and D Systems, af1700), Mouse anti-OCT4 (1:200; Santa Cruz Biotechnology, sc-5279) and Rabbit anti-GFP Alexa Fluor® 488 (1:100; Abcam, ab225314).
Article Title: The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200–dependent pathway in mice
Article Snippet: We purchased rabbit anti-CD133 (Abcam, ab19898), antiNotch1 (Abcam, ab27526), anti-Notch2 (Millipore, 07-1233), anti-Notch3 (Santa Cruz Biotechnology Inc., sc-5593), APC-conjugated anti-CD133 (eBioscience, 17-1331-81), goat anti-Jagged1 (Santa Cruz Biotechnology Inc., sc-6011), anti-actin (Santa Cruz Biotechnology Inc., sc-1616), goat anti-Delta (Santa Cruz Biotechnology Inc., sc-8155), rabbit anti-Delta3 (Santa Cruz Biotechnology Inc., sc-67269), rat anti-GATA1 (Santa Cruz Biotechnology Inc., sc-265), mouse anti-GATA2 (Santa Cruz Biotechnology Inc., sc-267), rabbit anti-GATA3 (Santa Cruz Biotechnology Inc., sc-9009), mouse anti-GATA4 (Santa Cruz Biotechnology Inc., sc-25310), rabbit anti-GATA6 (Santa Cruz Biotechnology Inc., sc-9055), goat anti-Zeb1 (Santa Cruz Biotechnology Inc., sc-10572), rabbit anti-Snai1 (Santa Cruz Biotechnology Inc., sc-28199), rabbit anti–N-cadherin (BD Transduction The Journal of Clinical Investigation http://www.jci.org Volume 121 Number 4 April 2011 1383 Laboratory, 610921), goat anti-Vimentin (Santa Cruz Biotechnology Inc., sc-5565), rabbit anti-phospho-SMAD2 (Cell Signaling Technology, 3108), anti-phospho-SMAD3 (Cell Signaling Technology, 9520), anti-total SMAD3 (Cell Signaling Technology, 9513), biotinylated anti-CD45 (BD Pharmingen, 553771), anti-CD31 (BD Pharmingen, 558737), and streptavidin-PE (BD Pharmingen, 554061) antibodies.
Article Title: The Notch ligand Jagged2 promotes lung adenocarcinoma metastasis through a miR-200-dependent pathway in mice
Article Snippet: We purchased rabbit anti-CD133 (Abcam, ab19898), anti-Notch1 (Abcam, ab27526), anti-Notch2 (Millipore, 07-1233), anti-Notch3 (Santa Cruz Biotechnology Inc., sc-5593), APC-conjugated anti-CD133 (eBioscience, 17-1331-81), goat anti-Jagged1 (Santa Cruz Biotechnology Inc., sc-6011), anti-actin (Santa Cruz Biotechnology Inc., sc-1616), goat anti-Delta (Santa Cruz Biotechnology Inc., sc-8155), rabbit anti-Delta3 (Santa Cruz Biotechnology Inc., sc-67269), rat anti-GATA1 (Santa Cruz Biotechnology Inc., sc-265), mouse anti-GATA2 (Santa Cruz Biotechnology Inc., sc-267), rabbit anti-GATA3 (Santa Cruz Biotechnology Inc., sc-9009), mouse anti-GATA4 (Santa Cruz Biotechnology Inc., sc-25310), rabbit anti-GATA6 (Santa Cruz Biotechnology Inc., sc-9055), goat anti-Zeb1 (Santa Cruz Biotechnology Inc., sc-10572), rabbit anti-Snai1 (Santa Cruz Biotechnology Inc., sc-28199), rabbit anti–N-cadherin (BD Transduction Laboratory, 610921), goat anti-Vimentin (Santa Cruz Biotechnology Inc., sc-5565), rabbit anti-phospho-SMAD2 (Cell Signaling Technology, 3108), anti-phospho-SMAD3 (Cell Signaling Technology, 9520), anti-total SMAD3 (Cell Signaling Technology, 9513), biotinylated anti-CD45 (BD Pharmingen, 553771), anti-CD31 (BD Pharmingen, 558737), and streptavidin-PE (BD Pharmingen, 554061) antibodies.
Immunofluorescence:Article Title: Gata2 specifies serotonergic neurons downstream of sonic hedgehog.
Article Snippet: .. Immunofluorescence on sections and wholemounts was performed as described (Hynes et al., 1995; Hynes et al., 2000), using rabbit anti5-HT (Serotec), rat anti-5-HT (Chemicon), mouse anti-3C2 (Developmental Studies Hybridoma Bank, University of Iowa), mouse anti-islet1 (Developmental Studies Hybridoma Bank, University of Iowa), mouse anti-Gata2 (Santa Cruz Biotech), mouse anti-Gata3 (Santa Cruz Biotech), mouse anti-Nkx2.2 (Developmental Studies Hybridoma Bank, University of Iowa) and rabbit anti-Nkx6.1 (Jensen et al., 1996). ..
Labeling:Article Title: GATA proteins identify a novel ventral interneuron subclass in the developing chick spinal cord.
Article Snippet: Briefly, the embryos were collected in ice-cold L-15 culture medium (GIBCO, Life Technology), fixed in 4% paraformaldehyde in 10 mM phosphate-buffered saline (PBS), and saturated with 30% sucrose in PBS overnight. .. Serial sections of 14- m thickness were then cut and labeled with following cell type-specific antibodies: mouse anti-GATA3 (Santa Cruz, sc-268), mouse anti-GATA2 (Santa Cruz, sc-269), mouse anti-Engrailed-1 [4G11; Developmental Study Hybridoma Bank (DSHB), University of Iowa], Enhb-1 (a gift of Dr. Alexandra Joyner, New York University, NY), rabbit anti-CHX10 (polyclonal antibody, a gift of Dr. Thomas Jessell, Columbia University, NY), mouse anti-ISLET1/2 (4D5 and 2D6; DSHB, University of Iowa), mouse anti-Sonic hedgehog (5E1; DSHB, University of Iowa), rabbit anti-NKX6.1 (polyclonal antibody; a gift of Dr. Martyn Goulding, The Salk Institute for Biological Studies, CA), and mouse anti-Tubulin antibody TUJ1. .. The primary antibodies were detected by using speciesspecific secondary antibodies conjugated with Cy3 (Jackson Laboratories) or BODIPY (Molecular Probes).
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